Ha Tag Chip Seq. In the current protocol, we describe a highly robust method f

In the current protocol, we describe a highly robust method for performing ChIP-seq of histone modifications in Arabidopsis thaliana plantlets. Either it was the antibody that I was using or the unsuitability inherent to the HA tag itself, I could never get any convincing data. Thermo Scientific Pierce Anti-HA Magnetic Beads are affinity particles for immunoprecipitation of recombinant HA-tagged proteins expressed in bacterial or mammalian cells. Learn more. This tag facilitates the detection, isolation, and purification of the proteins. (A) Peak calls in the Jan 6, 2008 · Combining this effort with genome-wide ChIP-chip or ChIP-Seq 11 analyses could facilitate the characterization of transcription factor–DNA interaction networks in mammalian cells, similar to 3 days ago · Protocol Specificity / Sensitivity HA-Tag (C29F4) Rabbit Monoclonal Antibody detects exogenously expressed proteins containing the HA epitope tag. Does the flag-tag influence a chip-seq experiment? I would perform a Chip-seq in cells overexpressing a tagged protein. By choosing our antibodies, you can trust in their specificity and consistency for identifying protein-DNA interactions. . , 2007). obd5btyuy
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